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Detalji o radu

Spectrophotometric Hybrid Flow System for Determination of N-Acetyl-L-Cysteine in Pharmaceuticals

AUTORI:

TOMISLAV KRALJEVIĆ;

ZNANSTVENA PODRUČJA
SADRŽAJ
A simple hybrid flow system is proposed for the determination of N-acetyl-L-cysteine based on the reaction between copper(II)-neocuproine and N-acetyl-L-cysteine, yielding an orange-yellow copper(I)-neocuproine complex with absorption maximum at 458 nm. The method involved injection of approximately 300 μL of sample/standard and 300 μL reagent solution simultaneously. The optimum conditions were evaluated, calibration curve was linear over the range of 1.0×10–6 - 1.0×10–4 mol L–1 of N-acetyl-L-cysteine with detection limit of 2.7 × 10–7 mol L–1. A sample throughput of 100 h−1 was obtained. This hybrid flow technique was found to be simple, accurate, reproducible and sensitive. The developed method was successfully applied for the determination of N-acetyl-L-cysteine in pharmaceutical preparations.
SADRŽAJ (eng)
A simple hybrid flow system is proposed for the determination of N-acetyl-L-cysteine based on the reaction between copper(II)-neocuproine and N-acetyl-L-cysteine, yielding an orange-yellow copper(I)-neocuproine complex with absorption maximum at 458 nm. The method involved injection of approximately 300 μL of sample/standard and 300 μL reagent solution simultaneously. The optimum conditions were evaluated, calibration curve was linear over the range of 1.0×10–6 - 1.0×10–4 mol L–1 of N-acetyl-L-cysteine with detection limit of 2.7 × 10–7 mol L–1. A sample throughput of 100 h−1 was obtained. This hybrid flow technique was found to be simple, accurate, reproducible and sensitive. The developed method was successfully applied for the determination of N-acetyl-L-cysteine in pharmaceutical preparations.
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IME PUBLIKACIJE